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| Home :: Services :: Contactual Research :: Bioactivity Guided Fractionation |
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| Bioactivity guided fractionation & isolation of active principles: |
Contractual Research, a wing of Natural Remedies is now offering, a service to isolate and identify bioactive constituents from herbal products. This new service has been the result of effective combination of Natural Remedies traditional strengths in phytochemistry with the battery of in-vitro bioassays (biochemical and cell based) standardized in-house.
Natural Remedies has taken up the initiative in isolating, purifying & characterizing over 200 different phytochemical constituents from important Indian medicinal plants to use as Primary Phytochemical Reference substance (www.phytocompounds.com). These reference substances have been characterized using chromatographic (TLC / HPTLC / HPLC), spectroscopic (UV, FTIR, NMR & Mass) and physico-chemical methods assuring their identity & purity. |
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| Typical steps involved in Bioactivity guided fractionation: |
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Finalization of the bioassay based on the end use of the product (for example, DPP IV inhibition assay for healthy blood sugar claims)
Fractionation of the extract / product which will be guided by bioassay
Isolation of bioactive constituent(s) from the active fractions
Characterization of bioactive constituent(s) by spectroscopic (NMR, IR etc,.) methods
Chromatographic method (HPLC/HPTLC etc.,) for estimation of bioactive constituents in the product
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| What do you get from Bioactivity guided fractionation ? |
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Identification of bioactive constituent(s) of the herbs being used in your product
Reference standard of the bioactive constituent(s) of your product
Chromatographic method for estimation of bioactive constituent(s) in your product (can be used for meaningful quality control)
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