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English
name: Pomegranate |
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Family:
Punicaceae |
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Part
used: Fruit-peel |
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Traditional
uses :
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Punica
is used in Mexican traditional medicine
for the treatment of gastrointestinal
disorders1. The peels are
powerful astringent and cure for diarrhea,
oral aphthae. Juice is a refrigerant
which lowers fever2 and it
is a blood tonic3. |
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Punica
granatum consists of glucosides,
tannin, ursolic acid, carbohydrates,
carotene, pectin, protein, riboflavin,
ellagic acid, gallic acid, glutamine,
tannins( like punicalgins and punicalin),
sitosterol and its ß-D-glucoside,etc. |
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Punica
granatum is reported to have antioxidant5-9,
anti-atherosclerotic10-12,
antibacterial13, anthelmintic14,
antiviral15. It is also used
in oral hygiene16, anti obesity17
and as an adjunct therapy to increase
bioavailability of radioactive dyes
during diagnostic imaging.18-19. |
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Antioxidant
and heart health.
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References: |
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| 1. |
Calzada
F,J Ethnopharmacol. 2006,108(3):367-70.
Epub. |
| 2. |
Arseculeratne, S.N., Gunatilaka,
A.A.L., Panabokke, R.G., 1985. Studies
on medicinal plants of Sri Lanka. |
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Part 14. Toxicity of some
traditional medicinal herbs. Journal of
Ethnopharmacology 13, 323–335. |
| 3. |
Lad,
V., Frawley, D., 1986. The Yoga of Herbs.
Lotus Press, Santa Fe, NM, pp. 135-136. |
| 4. |
Database on Medicinal
plants used in Ayurveda, Vol-2, New Delhi,
CCRAS, 2001.page no. 177. |
| 5. |
Reddy
MK, et al, Planta Med. 2007 ,73(5):461-7. |
| 6. |
Faria A et al, Eur J Nutr.
2007 ,46(5):271-8. |
| 7. |
Guo S et al, J Agric Food
Chem. 2007 ,18;55(8):3134-40. |
| 8. |
Rout S, Banerjee R, Bioresour
Technol. 2007, 98(16):3159-63. |
| 9. |
Ricci D et al, Fitoterapia.
2006 ,77(4):310-2. |
| 10. |
Aviram et al, Clinical
Nutrition, 23, 423 – 433 (2004). |
| 11. |
Kaplan et a.,. Biochem.
Mol. Act. Nutr. 2001 |
| 12. |
Napoli et al Proc. Natl.
Acad. Sci, 102, 4896 – 4901 (2005) |
| 13. |
Pérez, C. and Anesini,
C. (1994). In vitro antibacterial activity
of Argentinean folk medicinal plants |
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against Salmonella typhi,
J.Ethnopharmacol, 44, 41-46 |
| 14. |
Naovi, S.A.H., Khan, M.S.Y.
and Vohora, S.B. (1991). Antibacterial,
antifungal and anthelmintic |
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investigations on Indian
medicinal plants.Fitoterapia, 62,221-228. |
| 15. |
Zhang, J. (1995). Antiviral
activity of tannin from pericarp of Punica
granatum against genital |
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Herpes simplex virus in
vitro. China journal of Chinese material
Medica, 20,556-558. |
| 16. |
Menezes SM et al, 1: J
Herb Pharmacother. 2006;6(2):79-92. |
| 17. |
Lei F et al, Int
J Obes (Lond). 2007, 31(6):1023-9. |
| 18. |
Il'iasov, T.N., 1975. Vestnik
Rentgenologii i Radiologii 5, 83-84. |
| 19. |
Amorim, L.F et al,2003, Cellular and Molecular Biology 49,
501-507. |
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DOC
NO. |
: |
NR/QCD/SPC/126 |
| ISSUE
DATE |
: |
02-04-2007 |
| REVISION
NO. |
: |
00 |
| ANALYTICAL
SPECIFICATION |
NUMBER
OF PAGES |
: |
01 |
| TITLE
: PUNICA
GRANATUM EXTRACT
(³
10% PUNICALAGIN) |
| Plant
part |
: |
Fruit rind / Peel |
Fresh/Dry
|
: |
Dry |
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Sl. No. |
Tests |
Limits |
Protocol |
| 1.
|
Description |
Brown
to Dark brown powder |
| 2.
|
Identification |
To pass the test |
By HPLC |
| 3.
|
Physico-chemical
analysis |
< 7.0 |
As per USP <921> Method II |
| Moisture
(%w/w) |
| Acid
insoluble Ash (%w/w) |
< 3.0 |
As per USP<561> |
| Total
Ash %(w/w) |
< 10.0 |
| 4.
|
Particle
Size |
0.15 - 0.60 |
As per USP <616> Method -
I |
| Bulk
Density (g/cc) |
| Tapped
bulk density |
0.20 - 0.80 |
| Material passing through 30# BS/35 ASTM (%w/w) |
> 99.0 |
As per USP <786> Particle Size distribution. |
| 5.
|
Heavy
metal analysis |
< 10 ppm |
AAS / ICP -ES |
| Lead |
| Arsenic
|
< 2 ppm |
| Cadmium
|
< 1 ppm |
| Mercury |
< 0.1 ppm |
| 6.
|
Microbiological
analysis |
< 104 cfu g-1 |
As per WHO/PHARMA/92.559/Rev.1
Pg.49-52 / As per USP <61> & <62> |
| As per FIP Guidelines
|
| Total
Viable Aerobic Count |
| Total
Enterobacteriaceae |
< 102 org g-1 |
| Total
Fungal Count |
< 102 fs g-1 |
| 7.
|
Test
for Specific Pathogen |
|
| As per FIP Guidelines |
| E.coli (1g) |
Absent |
| Salmonella
Sp.
(10g) |
Absent |
| S.aureus (1g) |
Absent |
| 8.
|
Mycotoxin
analysis |
< 5 ppb |
As per USP Test for Aflatoxins |
| Aflatoxins
(B1 + B2 +
G1 + G2) |
| 9.
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Residual
solvent analysis |
< 3000 |
As per USP |
| As per ICH Guidelines |
| Methanol
(ppm) |
| 10.
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Pesticide
residue analysis |
To comply with USP |
As per AOAC / USP |
| As per USP & BP
Limits |
| Organochlorine
Pesticides |
| Organophosphorus
Pesticides |
| Synthetic
pyrethroids |
| 11.
|
Phytochemical
analysis |
³ 10.0 |
By HPLC |
| Punicalagin (%w/w) |
| Ellagic acid (% w/w) |
³ 2.5 |
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