| Two Week Training Module |
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| Section I: Overview of the essentials of research |
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This section gives an insight into the proper preparation and storage of buffers and reagents. Most laboratories lack formal programs to train students on proper technique of pipetting. This section also helps in developing operator pipetting proficiency and consistency in single-channel and multi-channel pipetting, to minimize pipetting errors.
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| Contents: |
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Instrument Handling |
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Reagent and buffer preparation |
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Pipetting skills and calibration |
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| Section II: In vitro screening with different platforms |
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In vitro screening involves testing the drug candidates on various targets. The screening involves target based assay development. The assays are based on various platforms or assay formats.
This course is designed to provide an understanding of target based drug screening based on two different platforms and the associated instruments. |
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| Spectrophotometry: |
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Operation, working principle and handling of Microplate reader. |
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Biossays in Kinetic and End point mode using Microplate reader. |
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Standardization of Bioassays. |
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Screening of herbal extracts in in vitro assays. |
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Data interpretation through sophisticated software programmes. |
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| Spectrofluorometry: |
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Operation, working principle and handling of Spectrofluorimeter (Fluostar) and the associated software programme. |
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Biossays in Kinetic and End point mode using FLUOstar. |
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Bioassay development and validation using assay performance measures |
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Screening inhibitors using different bioassay models |
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Bioassay miniaturization |
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Data interpretation through sophisticated software programmes |
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| Application Procedure |
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| Students interested to pursue this training programme can contact us at
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along with their profile. Any query in this regard will be entertained. |
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